dermal fibroblasts human primary foreskin dermal fibroblast cells Search Results


allogeneic neonatal fibroblasts vaveltatm  (Intercytex)
90
Intercytex allogeneic neonatal fibroblasts vaveltatm
Allogeneic Neonatal Fibroblasts Vaveltatm, supplied by Intercytex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dermal+fibroblasts+human+primary+foreskin+dermal+fibroblast+cells/pm20711212-4974-8-14?v=Intercytex
Average 90 stars, based on 1 article reviews
allogeneic neonatal fibroblasts vaveltatm - by Bioz Stars, 2026-07
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human neonatal foreskin dermal fibroblasts ds biomedical  (DS Pharma Biomedical)
90
DS Pharma Biomedical human neonatal foreskin dermal fibroblasts ds biomedical
Human Neonatal Foreskin Dermal Fibroblasts Ds Biomedical, supplied by DS Pharma Biomedical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dermal+fibroblasts+human+primary+foreskin+dermal+fibroblast+cells/pmc03732928-221-0-5?v=DS+Pharma+Biomedical
Average 90 stars, based on 1 article reviews
human neonatal foreskin dermal fibroblasts ds biomedical - by Bioz Stars, 2026-07
90/100 stars
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human dermal foreskin fibroblasts  (Lonza)
90
Lonza human dermal foreskin fibroblasts
Human Dermal Foreskin Fibroblasts, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dermal+fibroblasts+human+primary+foreskin+dermal+fibroblast+cells/pmc04966334-361-0-7?v=Lonza
Average 90 stars, based on 1 article reviews
human dermal foreskin fibroblasts - by Bioz Stars, 2026-07
90/100 stars
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human dermal fibroblasts derived neonatal foreskin (hdfs-neo  (Lonza)
90
Lonza human dermal fibroblasts derived neonatal foreskin (hdfs-neo
EV secretion was increased in replicative senescent <t>dermal</t> <t>fibroblasts.</t> ( A ) Senescence-associated (SA)-β-gal assay in young <t>HDFs</t> (PDL < 10) and senescent HDFs (PDL > 50). Scale bar = 50 μm. Data are means ± SD of three independent experiments on two independent senescent cell lines (*** p < 0.001). ( B ) Western blot analysis of p21 and p16 in young versus senescent HDFs. GAPDH was the loading control. ( C ) Levels of EVs derived from equal numbers of young and senescent HDFs. Protein concentrations in isolated EVs were determined by BCA assay. Data are means ± SD of four independent experiments using two independent senescent cell lines (*** p < 0.001). ( D ) Dynamic light scattering analysis of EVs derived from young and senescent HDFs. ( E ) Western blot analyses of EV markers. Five micrograms of EV protein were subjected to immunoblot analysis with anti-CD81, anti-CD9, anti-Alix, and anti-HSP90. GAPDH was the loading control.
Human Dermal Fibroblasts Derived Neonatal Foreskin (Hdfs Neo, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dermal+fibroblasts+human+primary+foreskin+dermal+fibroblast+cells/pmc07037765-144-10-19?v=Lonza
Average 90 stars, based on 1 article reviews
human dermal fibroblasts derived neonatal foreskin (hdfs-neo - by Bioz Stars, 2026-07
90/100 stars
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normal human foreskin dermal fibroblasts nhdfs  (LGC Promochem)
90
LGC Promochem normal human foreskin dermal fibroblasts nhdfs
EV secretion was increased in replicative senescent <t>dermal</t> <t>fibroblasts.</t> ( A ) Senescence-associated (SA)-β-gal assay in young <t>HDFs</t> (PDL < 10) and senescent HDFs (PDL > 50). Scale bar = 50 μm. Data are means ± SD of three independent experiments on two independent senescent cell lines (*** p < 0.001). ( B ) Western blot analysis of p21 and p16 in young versus senescent HDFs. GAPDH was the loading control. ( C ) Levels of EVs derived from equal numbers of young and senescent HDFs. Protein concentrations in isolated EVs were determined by BCA assay. Data are means ± SD of four independent experiments using two independent senescent cell lines (*** p < 0.001). ( D ) Dynamic light scattering analysis of EVs derived from young and senescent HDFs. ( E ) Western blot analyses of EV markers. Five micrograms of EV protein were subjected to immunoblot analysis with anti-CD81, anti-CD9, anti-Alix, and anti-HSP90. GAPDH was the loading control.
Normal Human Foreskin Dermal Fibroblasts Nhdfs, supplied by LGC Promochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dermal+fibroblasts+human+primary+foreskin+dermal+fibroblast+cells/10__3390_slash_molecules25184162-282-0-14?v=LGC+Promochem
Average 90 stars, based on 1 article reviews
normal human foreskin dermal fibroblasts nhdfs - by Bioz Stars, 2026-07
90/100 stars
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certified primary normal human dermal fibroblasts isolated from human male neonatal foreskin  (Lonza)
90
Lonza certified primary normal human dermal fibroblasts isolated from human male neonatal foreskin
EV secretion was increased in replicative senescent <t>dermal</t> <t>fibroblasts.</t> ( A ) Senescence-associated (SA)-β-gal assay in young <t>HDFs</t> (PDL < 10) and senescent HDFs (PDL > 50). Scale bar = 50 μm. Data are means ± SD of three independent experiments on two independent senescent cell lines (*** p < 0.001). ( B ) Western blot analysis of p21 and p16 in young versus senescent HDFs. GAPDH was the loading control. ( C ) Levels of EVs derived from equal numbers of young and senescent HDFs. Protein concentrations in isolated EVs were determined by BCA assay. Data are means ± SD of four independent experiments using two independent senescent cell lines (*** p < 0.001). ( D ) Dynamic light scattering analysis of EVs derived from young and senescent HDFs. ( E ) Western blot analyses of EV markers. Five micrograms of EV protein were subjected to immunoblot analysis with anti-CD81, anti-CD9, anti-Alix, and anti-HSP90. GAPDH was the loading control.
Certified Primary Normal Human Dermal Fibroblasts Isolated From Human Male Neonatal Foreskin, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dermal+fibroblasts+human+primary+foreskin+dermal+fibroblast+cells/pmc07358139-289-10-16?v=Lonza
Average 90 stars, based on 1 article reviews
certified primary normal human dermal fibroblasts isolated from human male neonatal foreskin - by Bioz Stars, 2026-07
90/100 stars
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primary human neonatal foreskin fibroblasts  (CELLnTEC Advanced Cell Systems AG)
90
CELLnTEC Advanced Cell Systems AG primary human neonatal foreskin fibroblasts
EV secretion was increased in replicative senescent <t>dermal</t> <t>fibroblasts.</t> ( A ) Senescence-associated (SA)-β-gal assay in young <t>HDFs</t> (PDL < 10) and senescent HDFs (PDL > 50). Scale bar = 50 μm. Data are means ± SD of three independent experiments on two independent senescent cell lines (*** p < 0.001). ( B ) Western blot analysis of p21 and p16 in young versus senescent HDFs. GAPDH was the loading control. ( C ) Levels of EVs derived from equal numbers of young and senescent HDFs. Protein concentrations in isolated EVs were determined by BCA assay. Data are means ± SD of four independent experiments using two independent senescent cell lines (*** p < 0.001). ( D ) Dynamic light scattering analysis of EVs derived from young and senescent HDFs. ( E ) Western blot analyses of EV markers. Five micrograms of EV protein were subjected to immunoblot analysis with anti-CD81, anti-CD9, anti-Alix, and anti-HSP90. GAPDH was the loading control.
Primary Human Neonatal Foreskin Fibroblasts, supplied by CELLnTEC Advanced Cell Systems AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dermal+fibroblasts+human+primary+foreskin+dermal+fibroblast+cells/pmc04637991-61-0-5?v=CELLnTEC+Advanced+Cell+Systems+AG
Average 90 stars, based on 1 article reviews
primary human neonatal foreskin fibroblasts - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
primary human foreskin fibroblasts  (CELLnTEC Advanced Cell Systems AG)
90
CELLnTEC Advanced Cell Systems AG primary human foreskin fibroblasts
EV secretion was increased in replicative senescent <t>dermal</t> <t>fibroblasts.</t> ( A ) Senescence-associated (SA)-β-gal assay in young <t>HDFs</t> (PDL < 10) and senescent HDFs (PDL > 50). Scale bar = 50 μm. Data are means ± SD of three independent experiments on two independent senescent cell lines (*** p < 0.001). ( B ) Western blot analysis of p21 and p16 in young versus senescent HDFs. GAPDH was the loading control. ( C ) Levels of EVs derived from equal numbers of young and senescent HDFs. Protein concentrations in isolated EVs were determined by BCA assay. Data are means ± SD of four independent experiments using two independent senescent cell lines (*** p < 0.001). ( D ) Dynamic light scattering analysis of EVs derived from young and senescent HDFs. ( E ) Western blot analyses of EV markers. Five micrograms of EV protein were subjected to immunoblot analysis with anti-CD81, anti-CD9, anti-Alix, and anti-HSP90. GAPDH was the loading control.
Primary Human Foreskin Fibroblasts, supplied by CELLnTEC Advanced Cell Systems AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dermal+fibroblasts+human+primary+foreskin+dermal+fibroblast+cells/pm21411248-57-0-7?v=CELLnTEC+Advanced+Cell+Systems+AG
Average 90 stars, based on 1 article reviews
primary human foreskin fibroblasts - by Bioz Stars, 2026-07
90/100 stars
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normal human dermal fibroblasts (nhdf) juvenile foreskin  (PromoCell)
N/A
Primary Normal Human Dermal Fibroblasts isolated from the dermis of juvenile foreskin or adult skin. Primary Normal Human Dermal Fibroblasts (NHDF) are isolated form the dermis of juvenile foreskin or adult skin from different locations
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Image Search Results


EV secretion was increased in replicative senescent dermal fibroblasts. ( A ) Senescence-associated (SA)-β-gal assay in young HDFs (PDL < 10) and senescent HDFs (PDL > 50). Scale bar = 50 μm. Data are means ± SD of three independent experiments on two independent senescent cell lines (*** p < 0.001). ( B ) Western blot analysis of p21 and p16 in young versus senescent HDFs. GAPDH was the loading control. ( C ) Levels of EVs derived from equal numbers of young and senescent HDFs. Protein concentrations in isolated EVs were determined by BCA assay. Data are means ± SD of four independent experiments using two independent senescent cell lines (*** p < 0.001). ( D ) Dynamic light scattering analysis of EVs derived from young and senescent HDFs. ( E ) Western blot analyses of EV markers. Five micrograms of EV protein were subjected to immunoblot analysis with anti-CD81, anti-CD9, anti-Alix, and anti-HSP90. GAPDH was the loading control.

Journal: International Journal of Molecular Sciences

Article Title: Extracellular Vesicles Derived from Senescent Fibroblasts Attenuate the Dermal Effect on Keratinocyte Differentiation

doi: 10.3390/ijms21031022

Figure Lengend Snippet: EV secretion was increased in replicative senescent dermal fibroblasts. ( A ) Senescence-associated (SA)-β-gal assay in young HDFs (PDL < 10) and senescent HDFs (PDL > 50). Scale bar = 50 μm. Data are means ± SD of three independent experiments on two independent senescent cell lines (*** p < 0.001). ( B ) Western blot analysis of p21 and p16 in young versus senescent HDFs. GAPDH was the loading control. ( C ) Levels of EVs derived from equal numbers of young and senescent HDFs. Protein concentrations in isolated EVs were determined by BCA assay. Data are means ± SD of four independent experiments using two independent senescent cell lines (*** p < 0.001). ( D ) Dynamic light scattering analysis of EVs derived from young and senescent HDFs. ( E ) Western blot analyses of EV markers. Five micrograms of EV protein were subjected to immunoblot analysis with anti-CD81, anti-CD9, anti-Alix, and anti-HSP90. GAPDH was the loading control.

Article Snippet: Human dermal fibroblasts derived from the skin of a 33-year old adult (HDFs) or neonatal foreskin (HDFs-Neo) were purchased (Lonza, Basel, Switzerland) and cultured in DMEM supplemented with 10% FBS (Lonza, Basel, Switzerland).

Techniques: Western Blot, Derivative Assay, Isolation, BIA-KA

EV biogenesis increased in senescent dermal fibroblasts. ( A ) Schematic image of sandwich ELISA using anti-CD81 antibody to detect EVs. ( B ) Conditioned media used to culture equal numbers of young and senescent HDFs were harvested at the indicated time points. EV levels were quantitated by sandwich ELISA for CD81. Data are means ± SD of three independent experiments using a senescent cell line (*** p < 0.001). ( C ) HDFs were labeled with a fluorescent lipid molecule N-Rh-PE (red) for the indicated time periods and co-stained with anti-CD63 antibody (green). Nuclei were stained with 4′6-diamidino-2-phenylindole (DAPI; blue). Fluorescence images were taken under a confocal microscope. Representative images are shown. Scale bar = 20 μm.

Journal: International Journal of Molecular Sciences

Article Title: Extracellular Vesicles Derived from Senescent Fibroblasts Attenuate the Dermal Effect on Keratinocyte Differentiation

doi: 10.3390/ijms21031022

Figure Lengend Snippet: EV biogenesis increased in senescent dermal fibroblasts. ( A ) Schematic image of sandwich ELISA using anti-CD81 antibody to detect EVs. ( B ) Conditioned media used to culture equal numbers of young and senescent HDFs were harvested at the indicated time points. EV levels were quantitated by sandwich ELISA for CD81. Data are means ± SD of three independent experiments using a senescent cell line (*** p < 0.001). ( C ) HDFs were labeled with a fluorescent lipid molecule N-Rh-PE (red) for the indicated time periods and co-stained with anti-CD63 antibody (green). Nuclei were stained with 4′6-diamidino-2-phenylindole (DAPI; blue). Fluorescence images were taken under a confocal microscope. Representative images are shown. Scale bar = 20 μm.

Article Snippet: Human dermal fibroblasts derived from the skin of a 33-year old adult (HDFs) or neonatal foreskin (HDFs-Neo) were purchased (Lonza, Basel, Switzerland) and cultured in DMEM supplemented with 10% FBS (Lonza, Basel, Switzerland).

Techniques: Sandwich ELISA, Labeling, Staining, Fluorescence, Microscopy

Senescent HDF-derived EVs attenuate the dermal effect on keratinocyte differentiation but evoke proinflammatory cytokine IL-6. Human epidermal keratinocytes (HEKs) in culture were treated with EVs derived from 5 × 10 5 young and senescent HDFs for 4 days. HEKs cultured without EV treatment for 4 d served as the control (-). Cells were analyzed for mRNA ( A ) and protein ( B ) expression of keratinocyte differentiation-related (KRT1, LOR) or barrier function-related (BLMH) markers by qRT-PCR and western blot analyses, respectively. The mRNA levels were normalized to those of RPL13A. GAPDH was the loading control for western blot analysis. KRT1, keratin 1; LOR, loricrin; BLMH, bleomycin hydrolase. ( C , D ) HEKs were treated with EVs derived from 5 × 10 5 young and senescent HDFs. Conditioned media at 24 h and 4 d post-treatment with EVs were harvested for the determination of secreted IL-8 ( C ) and IL-6 ( D ) levels, respectively, with specific ELISA kits. Data (in A , C , D ) are means ± SD of three independent experiments using two senescent cell lines (* p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant).

Journal: International Journal of Molecular Sciences

Article Title: Extracellular Vesicles Derived from Senescent Fibroblasts Attenuate the Dermal Effect on Keratinocyte Differentiation

doi: 10.3390/ijms21031022

Figure Lengend Snippet: Senescent HDF-derived EVs attenuate the dermal effect on keratinocyte differentiation but evoke proinflammatory cytokine IL-6. Human epidermal keratinocytes (HEKs) in culture were treated with EVs derived from 5 × 10 5 young and senescent HDFs for 4 days. HEKs cultured without EV treatment for 4 d served as the control (-). Cells were analyzed for mRNA ( A ) and protein ( B ) expression of keratinocyte differentiation-related (KRT1, LOR) or barrier function-related (BLMH) markers by qRT-PCR and western blot analyses, respectively. The mRNA levels were normalized to those of RPL13A. GAPDH was the loading control for western blot analysis. KRT1, keratin 1; LOR, loricrin; BLMH, bleomycin hydrolase. ( C , D ) HEKs were treated with EVs derived from 5 × 10 5 young and senescent HDFs. Conditioned media at 24 h and 4 d post-treatment with EVs were harvested for the determination of secreted IL-8 ( C ) and IL-6 ( D ) levels, respectively, with specific ELISA kits. Data (in A , C , D ) are means ± SD of three independent experiments using two senescent cell lines (* p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant).

Article Snippet: Human dermal fibroblasts derived from the skin of a 33-year old adult (HDFs) or neonatal foreskin (HDFs-Neo) were purchased (Lonza, Basel, Switzerland) and cultured in DMEM supplemented with 10% FBS (Lonza, Basel, Switzerland).

Techniques: Derivative Assay, Cell Culture, Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay